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THE FA CARLSBERG TROPHY
Second Round Qualifying REPLAYS
| Isthmian Prem | AFC Hornchurch | 4 | 0 | Whyteleafe | Isthmian One S |
| S League Prem | Banbury United | 1 | 3 | Godalming Town | Isthmian One S |
| Isthmian Prem | Billericay Town | 3 | 0 | Barton Rovers | S League One M |
| S League One M | Burnham | 2 | 1 | Croydon Athletic | Isthmian One S |
| S League Prem | Evesham U | 1 | 2 | Northwood | Isthmian One N |
| N Prem Prem | FC United | 1 | 0 | Lancaster City | N Prem Div One N |
| S League One SW | Hungerford T | 3 | 0 | Yate Town | S League One SW |
| N Prem Div One N | Mossley AFC | 3 | 1 | Brackley Town | S League Prem |
| N Prem Div One S | Quorn | 3 | 2 | Boston United | N Prem Prem |
| Isthmian Prem | Wealdstone | 2 | 1 | Harrow Borough | Isthmian Prem |
Second Round Qualifying Saturday 31 October 2009
| N Prem Div One N | AFC Fylde | 2 | 1 | Glapwell | N Prem Div One S |
| S League One M | Arlesey Town | 2 | 1 | Oxford City | S League Prem |
| S League One M | Barton Rovers | 2 | 2 | Billericay Town | Isthmian Prem |
| S League Prem | Bashley | 2 | 1 | Marlow | S League One M |
| Isthmian Prem | Bognor Regis Town | 0 | 2 | Maidstone United | Isthmian Prem |
| Isthmian Prem | Boreham Wood | 3 | 2 | Slough Town | S League One SW |
| N Prem Prem | Boston United | 0 | 0 | Quorn | N Prem Div One S |
| S League Prem | Brackley Town | 1 | 1 | Mossley AFC | N Prem Div One N |
| N Prem Prem | Buxton | 0 | 1 | Stourbridge | S League Prem |
| S League Prem | Cambridge City | 0 | 1 | Matlock Town | N Prem Prem |
| N Prem Div One S | Carlton Town | 0 | 3 | North Ferriby United | N Prem Prem |
| Isthmian Prem | Carshalton Athletic | 3 | 1 | AFC Totton | S League One SW |
| S League Prem | Chippenham Town | 4 | 1 | Tooting & Mitcham United | Isthmian Prem |
| Isthmian One N | Concord Rangers | 2 | 0 | Enfield Town | Isthmian One N |
| Isthmian One S | Croydon Athletic | 1 | 1 | Burnham | S League One M |
| Isthmian Prem | Dartford | 3 | 0 | Chipstead | Isthmian One S |
| S League Prem | Farnborough | 5 | 2 | Burgess Hill Town | Isthmian One S |
| Isthmian One S | Fleet Town | 0 | 2 | Ramsgate | Isthmian One S |
| N Prem Prem | Frickley Athletic | 0 | 3 | Guiseley AFC | N Prem Prem |
| Isthmian One S | Godalming Town | 1 | 1 | Banbury United | S League Prem |
| Isthmian Prem | Harrow Borough | 2 | 2 | Wealdstone | Isthmian Prem |
| S League One M | Hitchin Town | 3 | 1 | Gosport Borough | S League One SW |
| Isthmian Prem | Kingstonian | 4 | 2 | Hendon | Isthmian Prem |
| N Prem Div One N | Lancaster City | 3 | 3 | FC United Of Manchester | N Prem Prem |
| N Prem Div One N | Leigh Genesis | 4 | 1 | Skelmersdale United | N Prem Div One N |
| N Prem Prem | Nantwich Town | 4 | 1 | Leek Town | N Prem Div One S |
| Isthmian One N | Northwood | 0 | 0 | Evesham United | S League Prem |
| N Prem Div One N | Salford City | 5 | 3 | Clitheroe | N Prem Div One N |
| N Prem Div One S | Shepshed Dynamo | 0 | 5 | FC Halifax Town | N Prem Div One N |
| Isthmian Prem | Tonbridge Angels | 6 | 1 | Merstham | Isthmian One S |
| S League Prem | Truro City | 4 | 1 | Thatcham Town | S League One SW |
| N Prem Prem | Whitby Town | 0 | 2 | Kings Lynn | N Prem Prem |
| Isthmian One S | Whyteleafe | 1 | 1 | AFC Hornchurch | Isthmian Prem |
| N Prem Div One S | Willenhall Town | 0 | 6 | Nuneaton Town | S League Prem |
| N Prem Div One S | Witton Albion | 4 | 1 | Brigg Town | N Prem Div One S |
| S League One SW | Yate Town | 1 | 1 | Hungerford Town | S League One SW |